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Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts
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Stimulation of CFU-GM growth by fibroblastic conditioned medium from different cell origin

P Peulve1, E Summereau, J P Vannier

  • 1Laboratoire DIFEMA, Faculté de Médecine et de Pharmacie, Saint-Etienne du Rouvray, France.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|January 1, 1987
PubMed

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Cell Culture01:21

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media