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CaZAT11 Negatively Fine-Tunes Pepper Immunity to Ralstonia solanacearum by Repressing CaHDZ27.

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<i>CaGβ</i> Promotes <i>CaWRKY40</i> to Activate Immunity Against <i>Ralstonia solanacearum</i> but Disables It from Activating Thermotolerance.

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CaAIL1 Acts Positively in Pepper Immunity against Ralstonia solanacearum by Repressing Negative Regulators.

Yutong Zheng1,2,3, Shicong He1,2,3, Weiwei Cai1,2,3

  • 1Key Laboratory of Applied Genetics of Universities in Fujian Province, Fujian Agriculture and Forestry University, No. 15, Shang xia dian Road, Jianxin Town, Cangshan District, Fuzhou, Fujian 350002, China.

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Pepper plants

Keywords:
CaAIL1Ralstonia solanacearumImmuinityPepperRegulationTarget gene

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Area of Science:

  • Plant biology
  • Molecular genetics
  • Plant immunity

Background:

  • APETALA2 (AP2) transcription factors are crucial for plant development.
  • Their specific roles in plant immunity are not well understood.

Purpose of the Study:

  • To investigate the function of the AP2 transcription factor CaAIL1 in pepper immunity against Ralstonia solanacearum infection (RSI).

Main Methods:

  • Virus-induced gene silencing and transient overexpression for loss- and gain-of-function studies.
  • Chromatin immunoprecipitation sequencing (ChIP-seq) to identify CaAIL1 target genes.
  • ChIP-qPCR and electrophoretic mobility shift assays (EMSAs) for validation.

Main Results:

  • CaAIL1 expression is upregulated by RSI and positively regulates pepper immunity.
  • CaAIL1 directly represses genes encoding negative immune regulators (e.g., CaRAP2-7) and derepresses positive regulators (e.g., CaEAS).
  • An atypical EAR motif in CaAIL1 is essential for its immune function.

Conclusions:

  • CaAIL1 enhances pepper immunity against RSI by modulating the expression of immune regulator genes.
  • This regulation involves direct binding to cis-elements in the promoters of target genes.
  • The atypical EAR motif is critical for CaAIL1's role in plant defense.