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Related Experiment Video

Updated: Oct 22, 2025

Author Spotlight: Modeling an Aspect of Preeclampsia in Female Mice Using Hypoxic Human Placenta-Derived Small Extracellular Vesicles

Author Spotlight: Modeling an Aspect of Preeclampsia in Female Mice Using Hypoxic Human Placenta-Derived Small Extracellular Vesicles

Published on: January 26, 2024

Down-regulated placental miR-21 contributes to preeclampsia through targeting RASA1.

Kun Dong¹, Ying Hou², Na Zhang¹

¹Department of Obstetrics, Zibo Central Hospital, Zibo, Shandong, China.

Hypertension in Pregnancy

|August 31, 2021

Summary

MicroRNA-21 (miR-21) levels are decreased in preeclampsia, leading to increased RASA1 expression. This impacts trophoblast cell function, suggesting miR-21

Keywords:

Preeclampsia RASA1 miRNA-21 placenta pregnancy

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Last Updated: Oct 22, 2025

Author Spotlight: Modeling an Aspect of Preeclampsia in Female Mice Using Hypoxic Human Placenta-Derived Small Extracellular Vesicles

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Published on: January 26, 2024

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Published on: April 14, 2023

Trophoblast Cell Recovery from Angiogenesis-Tube Formation Assay for Differentiation Marker Expression Analysis

Trophoblast Cell Recovery from Angiogenesis-Tube Formation Assay for Differentiation Marker Expression Analysis

Published on: November 8, 2024

Area of Science:

Reproductive biology
Molecular genetics
Pathophysiology

Background:

Preeclampsia is a pregnancy complication characterized by high blood pressure.
The molecular mechanisms underlying preeclampsia pathogenesis remain incompletely understood.
MicroRNAs (miRNAs) are implicated in regulating placental development and function.

Purpose of the Study:

To investigate the role of miR-21 and its target RASA1 in preeclampsia.
To elucidate the molecular mechanisms by which miR-21 affects trophoblast cells.

Main Methods:

Human placental tissues from preeclamptic and normal pregnancies were analyzed.
Quantitative reverse transcription PCR (RT-qPCR) and Western blot were used to assess gene and protein expression.
Immunohistochemistry was employed for protein localization.
Cellular assays (proliferation, invasion, apoptosis) were performed in HTR-8/SVneo cells with miR-21 manipulation.
Dual-luciferase reporter assays identified direct miRNA-target interactions.

Main Results:

miR-21 expression was significantly decreased in preeclamptic placentas.
RASA1 expression was significantly increased in preeclamptic placentas.
miR-21 suppression in trophoblast cells upregulated RASA1, reduced proliferation, inhibited invasion, and promoted apoptosis.
miR-21 overexpression reversed these effects.
RASA1 was confirmed as a direct target of miR-21.

Conclusions:

miR-21 plays a crucial role in regulating trophoblast cell behavior.
The miR-21/RASA1 axis is implicated in the pathophysiology of preeclampsia.
Targeting the miR-21/RASA1 pathway may offer therapeutic potential for preeclampsia.