Murine colonization model by Campylobacter coli DSPV458.

Area of Science:

• Microbiology
• Animal Models
• Food Safety

Background:

• Thermotolerant Campylobacter species are significant in foodborne disease transmission to humans.
• Limited experimental models exist for Campylobacter coli (C. coli) colonization, particularly in non-disease-inducing contexts.
• Understanding C. coli intestinal colonization is crucial for controlling its spread in food-producing animals.

Purpose of the Study:

• To establish a murine experimental model for C. coli DSPV458 intestinal colonization.
• To assess the invasive capacity of C. coli DSPV458 in a host model.
• To provide a platform for evaluating interventions aimed at reducing C. coli intestinal loads.

Main Methods:

• Adult female Balb/cCmedc mice were divided into treated and control groups.
• Mice received antibiotics followed by oral gavage of C. coli DSPV458 in the treated group.
• Weekly necropsies and fecal cultures were performed to monitor colonization and translocation.

Main Results:

• C. coli DSPV458 was successfully isolated from all inoculated mice, confirming colonization.
• High levels of C. coli DSPV458 were quantified in feces, cecum, and ileum.
• No significant alterations in the clinical or physiological status of the mice were observed.

Conclusions:

• The developed murine model reliably establishes C. coli DSPV458 intestinal colonization.
• C. coli DSPV458 demonstrated no invasive capacity in this model.
• This model is suitable for evaluating strategies to reduce intestinal C. coli loads and developing alternatives to antibiotics for control in food animals.