Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cross-reactivity00:42

Cross-reactivity

31.8K
Overview
31.8K
Allergic Reactions02:06

Allergic Reactions

29.3K
Overview
29.3K
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

11.7K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
11.7K
Allergic Drug Reactions01:27

Allergic Drug Reactions

1.1K
Allergic reactions related to drugs are hypersensitivity responses driven by the immune system and bear no connection to the drug's therapeutic action. While drugs in isolation do not trigger an immune response, they can interact with endogenous proteins to form antigens. These antigens stimulate lymphocytes to produce antibodies. IgE-type antibodies attach themselves to mast cells. Upon subsequent exposure to the same stimulus, the antigen-antibody interaction is initiated, unleashing...
1.1K
Antibody Structure01:10

Antibody Structure

61.9K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
61.9K
Autoimmune Disorders01:29

Autoimmune Disorders

904
Autoimmune diseases are a group of disorders in which the body's immune system mistakenly attacks its own cells, tissues, and organs. This results from an overactive immune response against substances and tissues normally present in the body. Let's delve into the concept and mechanism of autoimmune diseases from an immune system point of view, explore different causes and examples of such diseases, and discuss potential solutions.
Concept and Mechanism of Autoimmune Diseases
The immune...
904

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prevalence of Avoidant/Restrictive Food Intake Disorder (ARFID) in children with and without food allergy.

Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology·2026
Same author

Nanocellulose Alleviates Intrahepatic Cholestasis of Pregnancy via Gut Microbiota-Mediated Bile Acid Homeostasis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Egg Allergy Prevalence Before and After Guidelines for Earlier Egg Introduction.

JAMA pediatrics·2026
Same author

Enrollment Assister Perspectives of a Private Health Insurance Program for Undocumented Immigrants.

JAMA health forum·2026
Same author

Reduced FBXO22 skews human trophoblast fate equilibrium toward syncytialization via polyubiquitinating the CoREST complex.

Nucleic acids research·2026
Same author

Asthma and allergy prevalence among children at risk for developmental coordination disorder.

Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology·2026

Related Experiment Video

Updated: Oct 22, 2025

Antigenic Liposomes for Generation of Disease-specific Antibodies
10:31

Antigenic Liposomes for Generation of Disease-specific Antibodies

Published on: October 25, 2018

12.5K

Epigenetic programming underpins B-cell dysfunction in peanut and multi-food allergy.

Samira Imran1, Melanie R Neeland1, Jennifer Koplin1

  • 1Murdoch Children's Research Institute, and Department of Paediatrics University of Melbourne Royal Children's Hospital Parkville VIC Australia.

Clinical & Translational Immunology
|September 1, 2021
PubMed
Summary

Epigenetic changes in B cells are linked to food allergy (FA). Distinct epigenetic profiles differentiate single-food from multi-food allergic individuals, revealing unique developmental pathways in persistent FA.

Keywords:
B cellsepigeneticsmulti‐food allergypeanut allergytranscriptomics

More Related Videos

Basophil Activation Test for Allergy Diagnosis
07:22

Basophil Activation Test for Allergy Diagnosis

Published on: May 31, 2021

8.7K
Humanized Mediator Release Assay as a Read-Out for Allergen Potency
10:22

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Published on: June 29, 2021

3.7K

Related Experiment Videos

Last Updated: Oct 22, 2025

Antigenic Liposomes for Generation of Disease-specific Antibodies
10:31

Antigenic Liposomes for Generation of Disease-specific Antibodies

Published on: October 25, 2018

12.5K
Basophil Activation Test for Allergy Diagnosis
07:22

Basophil Activation Test for Allergy Diagnosis

Published on: May 31, 2021

8.7K
Humanized Mediator Release Assay as a Read-Out for Allergen Potency
10:22

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Published on: June 29, 2021

3.7K

Area of Science:

  • Immunology
  • Epigenetics
  • Allergy Research

Background:

  • Food allergy (FA) rates are rising, with B-cell dysfunction implicated.
  • Epigenetic alterations in immune cells are increasingly recognized as contributors to FA.
  • Limited research has explored epigenetic changes specifically within B cells in FA.

Purpose of the Study:

  • To investigate epigenetic and transcriptomic profiles in B cells from adolescents with FA.
  • To compare these profiles between single-food-allergic, multi-food-allergic, and non-allergic individuals.
  • To identify epigenetic distinctions underlying different FA phenotypes.

Main Methods:

  • Purified B cells were obtained from adolescents with single-food FA, multi-food FA, and controls.
  • Epigenetic (DNA methylation) and transcriptomic (gene expression) analyses were performed.
  • Statistical and bioinformatic approaches were used to identify differentially methylated probes (DMPs) and differentially expressed genes (DEGs).

Main Results:

  • 144 DMPs and 116 DEGs distinguished FA B cells from controls, including methylation changes in the PM20D1 promoter.
  • 729 DMPs were specific to either single-food or multi-food allergic groups, indicating distinct epigenetic signatures.
  • Multi-food allergic individuals showed increased methylation near S100 genes and enriched myeloid cell activation terms in DEGs.

Conclusions:

  • Epigenetic modifications in B cells are associated with peanut allergy.
  • Distinct epigenetic features differentiate single-food and multi-food allergic individuals.
  • These findings suggest unique developmental pathways contributing to diverse FA phenotypes.