[Quality standard of Perillae Folium based on multicomponent determination with HPLC method]

Area of Science:

• Pharmacognosy
• Analytical Chemistry
• Natural Product Chemistry

Background:

• Perillae Folium is a traditional medicinal herb with various bioactive compounds.
• Accurate quantification of these compounds is essential for quality control and therapeutic efficacy.

Purpose of the Study:

• To establish and validate High-Performance Liquid Chromatography (HPLC) methods for determining five specific compounds in Perillae Folium.
• To analyze the content of these compounds across different batches of Perillae Folium.
• To identify potential quality markers for Perillae Folium.

Main Methods:

• Development of HPLC methods using a Kromasil C18 column.
• Optimization of mobile phases and gradient elution for separation of perillaketone, perillaldehyde, caffeic acid, scutellarin, and rosmarinic acid.
• Analysis of 33 batches of Perillae Folium.

Main Results:

• The established HPLC methods demonstrated good separation, linearity, and accuracy for the five target compounds.
• All 33 analyzed batches of Perillae Folium contained the five compounds, with varying concentrations.
• Rosmarinic acid, scutellarin, and perillaldehyde were found in higher concentrations than perillaketone and caffeic acid.
• The batches could be categorized into 5 clusters based on compound content and regional specificities.
• Perillaketone, perillaldehyde, and rosmarinic acid were identified as key markers for Perillae Folium quality assessment.

Conclusions:

• The developed HPLC methods are suitable for the quantitative determination of five key compounds in Perillae Folium.
• Compound profiling reveals significant variability among batches, suggesting regional influences.
• Perillaketone, perillaldehyde, and rosmarinic acid serve as reliable markers for evaluating the quality of Perillae Folium.