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Thermo-resistant enzyme-producing microorganisms isolated from composting.

S P M Cotta1, M S Marins2, I E Marriel3

  • 1Programa de Mestrado Profissional em Biotecnologia e Gestão da Inovação, Departamento de Ciências Biológicas, Centro Universitário de Sete Lagoas, UNIFEMM, Sete Lagoas, MG, Brasil.

Brazilian Journal of Biology = Revista Brasleira De Biologia
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Thermo-resistant microorganisms isolated from compost can serve as biological additives for organo-mineral fertilizers. These microbes exhibit high enzyme activity, offering a sustainable alternative to chemical fertilizers.

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Area of Science:

  • Microbiology
  • Biotechnology
  • Agricultural Science

Background:

  • Chemical fertilizers pose environmental concerns.
  • Organo-mineral fertilizers supplemented with biological additives offer a sustainable alternative.
  • Identifying robust microorganisms for such applications is crucial.

Purpose of the Study:

  • To isolate and identify thermoresistant microorganisms from composting mass.
  • To evaluate the enzymatic activities (amylase, phytase, cellulase) of these isolates.
  • To assess their potential as biological additives in organo-mineral fertilizers.

Main Methods:

  • Two-step isolation of thermoresistant microorganisms using heat shock and in vitro culture.
  • Molecular identification via partial sequencing of the 16S ribosomal gene.
  • Enzyme activity assays (amylase, phytase, cellulase) and calculation of enzymatic index (EI).

Main Results:

  • Forty-one thermoresistant isolates were obtained, with 35 identified as bacteria (primarily Bacillus species) and 2 as fungi.
  • Thirty-eight of the 41 isolates produced at least one enzyme.
  • High amylase, phytase, and cellulase activities were recorded in several Bacillus and Brevibacillus species.

Conclusions:

  • Selected thermoresistant microorganisms, particularly Bacillus species, demonstrate significant enzymatic potential.
  • These microbes are promising candidates for biological additives in organo-mineral fertilizers.
  • The study supports the use of microbial consortia for sustainable agricultural practices.