Related Experiment Video
Updated: Oct 21, 2025
04:25
Capsular Serotyping of Streptococcus pneumoniae Using the Quellung Reaction
Published on: February 24, 2014
46.0K
Triplex Direct Quantitative Polymerase Chain Reaction for the Identification of Streptococcus pneumoniae Serotypes
Mahamoudou Ouattara1, Mamadou Tamboura2, Dinanibè Kambiré2
1Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
The Journal of Infectious Diseases
|September 1, 2021
Abstract
None:
The quantitative polymerase chain reaction (qPCR) method presented in this study allows the identification of pneumococcal capsular serotypes in cerebrospinal fluid without first performing DNA extraction. This testing approach, which saves time and resources, demonstrated similar sensitivity and a high level of agreement between cycle threshold values when it was compared side-by-side with the standard qPCR method with extracted DNA.
