Related Experiment Video

Updated: Oct 21, 2025

Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue
04:41

Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue

Published on: July 28, 2023

2.8K

Persistent JunB activation in fibroblasts disrupts stem cell niche interactions enforcing skin aging

Pallab Maity1, Karmveer Singh1, Linda Krug2

  • 1Department of Dermatology and Allergic Diseases, Ulm University, 89081 Ulm, Germany; Aging Research Center (ARC), 89081 Ulm, Germany.

Cell Reports
|September 1, 2021
PubMed

Insights

Researchers discovered that the transcription factor JunB drives fibroblast senescence, leading to skin aging. Silencing JunB rejuvenates skin stem cells and restores tissue integrity, offering targets for anti-aging therapies.

Area of Science:

  • Cellular Biology
  • Dermatology
  • Aging Research

Background:

  • Fibroblasts in connective tissue are crucial for stem cell niches, especially in skin.
  • The mechanisms by which fibroblasts influence organ aging are not fully understood.

Purpose of the Study:

  • To elucidate the mechanisms of fibroblast senescence and its impact on skin aging.
  • To identify JunB as a key regulator of fibroblast senescence and connective tissue niche integrity.

Main Methods:

  • Investigated the role of redox-dependent activation of transcription factor JunB.
  • Analyzed the upregulation of p16INK4A and repression of insulin-like growth factor-1 (IGF-1).
  • Examined the effects of JunB silencing on skin stem cell pools and tissue integrity.

Main Results:

  • JunB activation leads to fibroblast senescence via p16INK4A upregulation and IGF-1 repression.
  • Fibroblast senescence disrupts the niche, depleting stem cell pools and causing skin decline.
  • Silencing JunB restores IGF-1 and p16 levels, rejuvenating stem cells and skin tissue.

Conclusions:

  • JunB is a critical regulator of the connective tissue niche and fibroblast senescence.
  • Targeting JunB offers a potential therapeutic strategy for combating skin aging and related conditions.
Keywords:
IGF-1JunBagingfibroblastsp16(INK4A)redox imbalanceskinstem cell niche

More Related Videos

Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo09:19

Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo

Published on: February 15, 2021

5.1K
Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting09:14

Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting

Published on: May 7, 2019

16.2K

Related Concept Videos

Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...
2.7K
Tissue Renewal without Stem Cells01:23

Tissue Renewal without Stem Cells

After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...
1.9K
Stem Cell Niche01:26

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
5.5K
Multipotency and Niche of Bulge Stem Cell01:06

Multipotency and Niche of Bulge Stem Cell

A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...
3.9K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.2K
Introduction to Fibroblasts01:09

Introduction to Fibroblasts

Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...
3.3K