A mobile angular scattering microscope for organelle size estimation

Angular light scattering measurements have been used to determine the size parameters of spherical particles. By measuring the angular scattering from biological specimen, the average size of the cellular organelles can be estimated, which can be used to determine information about the health of the biological sample. An angular scattering microscope with the ability to be easily moved was constructed from common inexpensive components, which has potential applications for clinical and low-resource settings. The stability and accuracy of the system were investigated by measuring the scattering from polystyrene beads with mean sizes of 5 and 1.75 μm with narrow size distributions. Resulting size estimates obtained from the scattering patterns were consistent with the manufacturer-specified range of diameters for each sample. Initial studies were also conducted on individual fixed HeLa cells. The results presented indicate that the system is capable of obtaining precise and accurate size estimates of beads and single cells' organelles.