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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Related Experiment Video

Updated: Oct 21, 2025

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

Custom long non-coding RNA capture enhances detection sensitivity in different human sample types.

Annelien Morlion^1,2, Celine Everaert^1,2, Justine Nuytens^1,2

¹OncoRNALab, Center for Medical Genetics, Department of Biomolecular Medicine, Ghent University, B-9000 Ghent, Belgium.

|September 2, 2021

Summary

This summary is machine-generated.

This study introduces a novel long non-coding RNA (lncRNA) capture sequencing method. This approach enhances the detection of lncRNAs in complex samples, improving diagnostic and therapeutic target identification.

Keywords:

FFPE RNA abundance RNA expression RNA sequencing biofluid lncRNA lncRNome probes

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Last Updated: Oct 21, 2025

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Published on: July 9, 2021

Area of Science:

Molecular Biology
Genomics
Biotechnology

Background:

Long non-coding RNAs (lncRNAs) are crucial regulators in cellular processes.
lncRNAs exhibit cell- and cancer-specific expression, making them valuable diagnostic and therapeutic targets.
Characterizing lncRNAs in bulk transcriptome data is difficult due to their low abundance.

Purpose of the Study:

To develop and evaluate a custom lncRNA capture sequencing approach.
To improve the detection and characterization of lncRNAs in diverse biological samples.

Main Methods:

Designed a custom capture sequencing method utilizing 565,878 probes for 49,372 human lncRNA genes.
Validated the approach on various sample types, including RNA mixtures, FFPE tissues, and biofluids.

Main Results:

The custom lncRNA capture method significantly enhanced lncRNA detection compared to standard RNA sequencing.
Demonstrated improved reproducibility and higher coverage for lncRNA identification.
Successfully characterized lncRNAs in challenging sample types like FFPE tissues and biofluids.

Conclusions:

The custom lncRNA capture sequencing approach offers a powerful tool for comprehensive lncRNA analysis.
This method facilitates the discovery of novel diagnostic and therapeutic targets through improved lncRNA characterization.