Related Experiment Video

Updated: Oct 21, 2025

Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans
11:26

Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans

Published on: February 7, 2018

11.8K

Extremely Bright, Near-IR Emitting Spontaneously Blinking Fluorophores Enable Ratiometric Multicolor Nanoscopy in

Jonathan Tyson1,2, Kevin Hu3,4, Shuai Zheng1

  • 1Department of Chemistry, University of California, Berkeley, California 94720, United States.

ACS Central Science
|September 2, 2021
PubMed

More Related Videos

Conducting Multiple Imaging Modes with One Fluorescence Microscope08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

10.0K
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.1K

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
10.5K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
9.4K