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Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
Published on: September 12, 2019
Secondary Structure Analysis of Single Silk Nanofibril through Infrared Nanospectroscopy
Yawen Liu1, Hongchong Guo1, Shengjie Ling2
1School of Physical Science and Technology, ShanghaiTech University, Shanghai, China.
Abstract:
Infrared nanospectroscopy (NanoIR) is a new experimental technique to research the secondary structure of protein-based nanoarchitectures in recent years. Compared with the conventional IR, NanoIR reveals to be an exquisite, sensitive, and accurate tool to analyze and image the single molecule secondary structure, which can reach up to high spatial resolution (10 nm). Here we present a detailed protocol to introduce how to study single silk nanofibril (SNF) and process the results by this routine. This protocol provides a useful method to demonstrate the microstructure of nanomaterials by NanoIR, displaying the potential application in analytical chemistry, biomaterials, and nanotechnologies.
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