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Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast
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Endosomal sorting complexes required for transport-0 (ESCRT-0) are essential for fungal development, pathogenicity,

Li-Xiao Sun1, Hui Qian1, Meng-Yu Liu1

  • 1State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-products, Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, China.

Environmental Microbiology
|September 2, 2021
PubMed

Insights

The ESCRT-0 complex, comprising Hse1 and Vps27, is crucial for regulating the growth, virulence, and essential cellular processes like autophagy and ER-phagy in the rice blast fungus Magnaporthe oryzae.

Area of Science:

  • Molecular Mycology
  • Cell Biology
  • Plant Pathology

Background:

  • Magnaporthe oryzae causes devastating rice blast disease.
  • ESCRT-0 complex (Hse1, Vps27) is vital for multivesicular body (MVB) biogenesis.
  • ESCRT-0 functions in M. oryzae remain largely uncharacterized.

Purpose of the Study:

  • To identify and functionally characterize Hse1 and Vps27 in M. oryzae.
  • To elucidate the role of ESCRT-0 in fungal development and pathogenicity.
  • To investigate ESCRT-0 involvement in autophagy and ER homeostasis.

Main Methods:

  • Gene disruption of MoHse1 and MoVps27.
  • Phenotypic analysis of mutant strains (growth, conidiation, development, pathogenicity).
  • Analysis of autophagy markers (MoAtg8 lipidation, GFP-MoAtg8 degradation) and ER stress responses (UPR, ER-phagy).

Main Results:

  • Disruption of MoHse1 and MoVps27 led to severe defects in growth, development, and pathogenicity, causing loss of virulence.
  • ESCRT-0 mutants exhibited dysregulated autophagy, evidenced by altered MoAtg8 lipidation and degradation.
  • ESCRT-0 interacts with COPII, and its disruption impaired UPR and ER-phagy.

Conclusions:

  • ESCRT-0 is essential for M. oryzae development, virulence, and maintaining cellular homeostasis.
  • ESCRT-0 regulates autophagy and ER-phagy pathways in M. oryzae.
  • Targeting ESCRT-0 could be a strategy to control rice blast disease.

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