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Versatile microporous polymer-based supports for serial macromolecular crystallography.

Isabelle Martiel1, John H Beale1, Agnieszka Karpik1

  • 1Paul Scherrer Institute, Forschungsstrasse 111, 5232 Villigen, Switzerland.

Acta Crystallographica. Section D, Structural Biology
|September 2, 2021
PubMed
Summary

Researchers developed a novel polymer membrane support for serial data collection at advanced light sources. This method simplifies sample handling and enhances data quality for challenging protein crystals.

Keywords:
SFXfixed targetsample supportsserial crystallography

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Area of Science:

  • Structural Biology
  • Biophysics
  • Materials Science

Background:

  • Serial data collection is crucial for state-of-the-art light sources like synchrotrons and X-ray free-electron lasers.
  • Challenging samples, including small crystals with weak diffraction and low-dose limits, greatly benefit from serial data collection methods.

Purpose of the Study:

  • To demonstrate the utility of a thin polymer-based membrane as a support for serial data collection and screening experiments.
  • To evaluate the suitability of these supports for various protein crystals, including those in challenging matrices.

Main Methods:

  • Development and application of a thin polymer-based membrane support for sample preparation.
  • Testing the membrane supports with a diverse range of protein crystals suspended in liquids.
  • Evaluating the performance of the supports for challenging samples like membrane proteins in sponge phase.

Main Results:

  • The polymer membrane supports are suitable for a wide array of protein crystals in liquid suspensions.
  • The supports are effective for challenging samples, such as membrane proteins in sponge phase.
  • The sample deposition method is simple, robust, flexible, and adaptable, yielding thin specimens with low background and minimal mother liquor.

Conclusions:

  • The demonstrated polymer membrane supports offer a simple, robust, and adaptable method for serial data collection.
  • These supports are effective for various protein crystals and challenging applications, including membrane proteins.
  • Affordable fabrication makes these supports a promising option for serial experiments at synchrotrons and X-ray free-electron lasers.