Tb(III)-DO3A and BODIPY dyad as multimode responsive hypochlorite probe

Area of Science:

• Analytical Chemistry
• Chemical Sensing
• Biomedical Imaging

Background:

• Hypochlorite (ClO-) is a reactive oxygen species involved in biological processes and oxidative stress.
• Developing sensitive and selective probes for hypochlorite detection is crucial for biological and environmental monitoring.
• Existing probes often lack multimode detection capabilities, limiting their application versatility.

Purpose of the Study:

• To develop a novel, multimode responsive probe for hypochlorite detection.
• To evaluate the probe's performance in various detection modes, including colorimetric, fluorogenic, and phosphorescent.
• To demonstrate the probe's utility in practical applications such as naked-eye detection, test paper development, and in vitro cellular imaging.

Main Methods:

• Synthesis of a novel probe integrating Terbium (III)-1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid (Tb(III)-DO3A) and BODIPY.
• Spectroscopic analysis (absorption, fluorescence, phosphorescence) to characterize probe response to hypochlorite.
• Colorimetric and fluorogenic assays for detecting sodium hypochlorite (NaOCl) in solution.
• Fabrication of a colorimetric test paper for visual NaOCl sensing.
• In vitro fluorescence imaging studies in living cells to assess cellular detection capabilities.

Main Results:

• The developed probe exhibits simultaneous absorption, fluorescence, and phosphorescence responses to hypochlorite.
• The probe allows for naked-eye colorimetric and fluorogenic detection of NaOCl in solution.
• A functional colorimetric test paper was successfully constructed for visual NaOCl sensing.
• In vitro fluorescence imaging demonstrated the probe's effectiveness for detecting NaOCl in living cells.
• Probe 1 represents a rare example of a multimode responsive hypochlorite probe.

Conclusions:

• The novel multimode probe offers a versatile platform for hypochlorite detection.
• Its ability to respond via multiple spectroscopic channels enhances reliability and applicability.
• The probe shows significant potential for diverse applications, from simple visual tests to advanced cellular imaging.