Next step in 2nd generation glucose biosensors: Ferrocene-loaded electrospun nanofibers

Glucose determination is one of the most common analyses in clinical chemistry. Employing biosensors for this purpose has become the method of choice for home use for diabetic patients. To limit the impact of dissolved O₂ concentration or possible interferences (known hindrances in the classical glucose detection approach), a variety of mediated pathways have been explored. Herein, an ingenious, facile and low-cost approach for immobilization of redox mediator within nanofibrous mats is presented. This '2^nd generation' biosensor is able to avoid common issues such as leaching or diffusion barriers whilst providing the necessary close contact between the enzyme and the redox shuttle, for enhancing the detection accuracy and accelerate the response. Polyacrylonitrile nanofibers loaded with carbon nanotubes and ferrocene (PAN/Fc/MWCNT-COOH NFs) have been successfully prepared and applied as biosensing matrices upon cross-linking of glucose oxidase (GOD). The morphology of the NFs was investigated by means of scanning electron microscopy (SEM-EDX) and correlated to the kinetics of mediated electron transfer and to the efficiency in glucose detection, which were evaluated through cyclic voltammetry (CV) and amperometric measurements. The content of Fc was varied from 0.5 to 5.0 wt%, with optimum biosensor performance at 1.0 wt% exhibiting a linear range up to 8.0 × 10^-3 M with sensitivity of ~27.1 mAM^-1 cm^-2 and 4.0 μM LOD. Excellent stability (RSD 2.68%) during 40 consecutive measurements along with insignificant interference and accurate recovery in real sample analysis (~100%) make for a very reliable sensor that can easily render itself to miniaturization and has the potential for a wide range of practical applications.