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Updated: Oct 21, 2025
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Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization
Published on: April 19, 2015
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Controlling cell elongation and orientation by using microstructural nanofibre scaffolds for accelerating tissue
Nur Adila Mohd Razali1, Wei-Chih Lin1, Norul Ashikin Norzain1
1Department of Mechanical and Electro-mechanical Engineering, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.
Summary
Engineered triangular microstructured scaffolds guide cell alignment and significantly enhance tissue regeneration. These scaffolds show great potential for accelerating wound healing and promoting tissue repair.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Scaffold surface topography influences cellular behavior and tissue regeneration.
- Microstructured surfaces offer potential for guiding cell responses in wound healing.
Purpose of the Study:
- To engineer and evaluate microstructured scaffolds for enhanced tissue regeneration.
- To investigate the effect of triangular microstructures on cell morphology and alignment.
- To assess the efficacy of triangular microstructured scaffolds in wound healing.
Main Methods:
- Fabrication of silicon molds with microstructures (hemisphere, pyramid, semi-cylinder, triangle prism) using microfabrication.
- Transfer of microstructural patterns onto polycaprolactone membranes and nanofibrous scaffolds via molding and electrospinning.
- In vitro studies with C2C12 myoblast cells to assess cell morphology and alignment.
- In vivo wound healing studies to evaluate scaffold performance.
Main Results:
- Triangular microstructured nanofibrous scaffolds effectively guided C2C12 cell elongation and alignment (70% alignment, 4-fold elongation).
- Cells cultured on triangular scaffolds achieved an aspect ratio of 17.33 after 24 hours.
- In vivo studies demonstrated 95.04% wound closure within 14 days with ordered collagen arrangement.
Conclusions:
- Engineered triangular microstructured nanofibrous scaffolds promote significant cell alignment and elongation.
- These scaffolds accelerate wound healing and facilitate organized tissue regeneration.
- Triangular microstructured scaffolds hold promise for advanced wound healing applications.
