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Updated: Oct 21, 2025

Visualizing Methane-Cycling Microbial Dynamics in Coastal Wetlands
Published on: January 31, 2025
Methyloradius palustris gen. nov., sp. nov., a methanol-oxidizing bacterium isolated from snow
Takeshi Miyadera1,2, Hisaya Kojima3, Manabu Fukui2
1Graduate School of Environmental Science, Hokkaido University, Kita-10, Nishi-5, Kita-ku, Sapporo, 060-0810, Japan.
Abstract:
A novel methylotrophic bacterium, strain Zm11T, was isolated from reddish brown snow collected in a moor in Japan. Cells of the isolate were Gram-stain-negative, motile, and rod-shaped (0.6-0.7 × 1.2-2.7 μm). Growth was observed at 5-32 °C with an optimum growth temperature of 25-28 °C. The pH range for growth was 5.4-7.8 with an optimum pH of 6.8. The strain utilized only methanol as carbon and energy sources for aerobic growth. The major cellular fatty acids (> 40% of total) were summed feature 3 (C16:1ω7c and/or C16:1ω6c) and C16: 0. The predominant quinone was Q-8, and major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The complete genome of strain Zm11T is composed of a circular chromosome (2,800,413 bp), with G + C content of 46.4 mol%. Phylogenetic analyses were conducted based on the 16S rRNA gene sequence and conserved proteins encoded in the genome. The results of analyses indicate that strain Zm11T is a member of the family Methylophilaceae but does not belong to any existing genus. On the basis of its genomic and phenotypic properties, strain Zm11T (= DSM111909T = NBRC114766T) is proposed as the type strain of a new species in a new genus, Methyloradius palustris gen. nov., sp. nov.
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