Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Modelling arrhythmogenic cardiomyopathy fattyfibro pathology with PKP2-deficient epicardial cells derived from human iPSCs.

Communications biology·2025
Same author

Minimal Component, Protein-Free, and Cost-effective Human Pluripotent Stem Cell Cardiomyocyte Differentiation.

Current protocols·2025
Same author

Atrial Fibrillation Related Titin Truncation Is Associated With Atrial Myopathy in Patient-Derived Induced Pluripotent Stem Cell Disease Models.

Circulation. Genomic and precision medicine·2025
Same author

Leiomodin 2 neonatal dilated cardiomyopathy mutation results in altered actin gene signatures and cardiomyocyte dysfunction.

NPJ Regenerative medicine·2024
Same journal

Photobiomodulation Targets Mitochondrial Homeostasis for Diabetic Wound Healing.

Tissue engineering. Part B, Reviews·2026
Same journal

Nanotechnology-Driven Noninvasive and Targeted Therapies for Anaplastic Thyroid Carcinoma.

Tissue engineering. Part B, Reviews·2026
Same journal

From Restoration to Regeneration: A Review of 3D Printing Strategies in Digital Dentistry.

Tissue engineering. Part B, Reviews·2026
Same journal

Molecular Pathogenesis of and Regenerative Strategies for Osteonecrosis of the Femoral Head.

Tissue engineering. Part B, Reviews·2026
Same journal

Regenerative Potential of Platelet-Rich Plasma-Loaded Scaffolds in Endometrial Damage: A Meta-Analysis.

Tissue engineering. Part B, Reviews·2026
Same journal

Enhancing Round Window Membrane Permeability for Inner Ear Drug Delivery: A Systematic Review.

Tissue engineering. Part B, Reviews·2026
See all related articles

Related Experiment Video

Updated: Oct 21, 2025

A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation
06:57

A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation

Published on: August 5, 2018

9.1K

Tissue Engineering Techniques for Induced Pluripotent Stem Cell Derived Three-Dimensional Cardiac Constructs.

Tori Salem1, Zachary Frankman2, Jared M Churko1,2

  • 1Department of Cellular and Molecular Medicine, University of Arizona Health Sciences, Tucson, Arizona, USA.

Tissue Engineering. Part B, Reviews
|September 3, 2021
PubMed
Summary
This summary is machine-generated.

This review details key factors for developing cardiovascular tissues from human stem cell-derived cardiomyocytes. It covers construct types, cell sources, and fabrication methods for 3D cardiac tissue engineering.

Keywords:
EHTscardiac tissueiPSCsorganoidstissue engineering

More Related Videos

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells
10:37

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells

Published on: March 14, 2021

6.9K
Preparation of Mesh-Shaped Engineered Cardiac Tissues Derived from Human iPS Cells for In Vivo Myocardial Repair
05:05

Preparation of Mesh-Shaped Engineered Cardiac Tissues Derived from Human iPS Cells for In Vivo Myocardial Repair

Published on: June 9, 2020

5.6K

Related Experiment Videos

Last Updated: Oct 21, 2025

A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation
06:57

A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation

Published on: August 5, 2018

9.1K
Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells
10:37

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells

Published on: March 14, 2021

6.9K
Preparation of Mesh-Shaped Engineered Cardiac Tissues Derived from Human iPS Cells for In Vivo Myocardial Repair
05:05

Preparation of Mesh-Shaped Engineered Cardiac Tissues Derived from Human iPS Cells for In Vivo Myocardial Repair

Published on: June 9, 2020

5.6K

Area of Science:

  • Cardiovascular Physiology
  • Developmental Biology
  • Biomedical Engineering

Background:

  • Human stem cell-derived cardiomyocytes are established models for cardiac physiology research.
  • Tissue-engineered human heart constructs are increasingly used for drug screening and disease modeling.
  • Cardiac tissue engineering integrates complex engineering and physiological principles, posing accessibility challenges.

Purpose of the Study:

  • To provide a comprehensive review of major considerations for developing cardiovascular tissues from stem cell-derived cardiomyocytes.
  • To examine recent advancements in cardiac tissue engineering.
  • To discuss essential concepts and principles for creating and analyzing 3D cardiac constructs.

Main Methods:

  • Review of literature on stem cell-derived cardiomyocytes and cardiac tissue engineering.
  • Discussion of various tissue-engineered construct formats.
  • Analysis of cardiomyocyte sources, fabrication techniques, and maturation processes for 3D constructs.

Main Results:

  • Human induced pluripotent stem cell-derived cardiomyocytes are becoming more cost-effective and routine.
  • Three-dimensional (3D) tissue-engineered constructs are replacing 2D monolayer cultures due to better representation of heart geometry.
  • Key principles for generating and interpreting data from 3D cardiac tissue models are presented.

Conclusions:

  • Advancements in stem cell technology facilitate the routine production of cardiomyocytes for research.
  • 3D cardiac tissue engineering offers more physiologically relevant models compared to traditional 2D cultures.
  • This review aims to enhance accessibility and understanding of 3D cardiac tissue engineering principles and applications.