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Tetrandrine suppresses the growth of human osteosarcoma cells by regulating multiple signaling pathways

Nan Wang1, Shengdong Yang2, Tao Tan3

  • 1Department of Orthopedics, The Third Affiliated Hospital of Chongqing Medical University, Chongqing, P.R. China.

Bioengineered
|September 3, 2021
PubMed

Insights

Tetrandrine (TET) effectively inhibits osteosarcoma (OS) cell growth, migration, and invasion while promoting apoptosis. This Chinese medicine targets multiple signaling pathways, showing promise for OS treatment.

Area of Science:

  • Oncology
  • Pharmacology
  • Molecular Biology

Background:

  • Osteosarcoma (OS) is the most common pediatric bone cancer with limited treatment advancements.
  • Tetrandrine (TET), a traditional Chinese medicine, exhibits anticancer properties, but its mechanisms in OS are not fully elucidated.

Purpose of the Study:

  • To investigate the efficacy and underlying mechanisms of Tetrandrine (TET) in treating osteosarcoma (OS).

Main Methods:

  • In vitro assays assessed TET's effects on OS cell proliferation, migration, invasion, and apoptosis.
  • Luciferase reporter and Western blotting assays identified targeted signaling pathways.
  • In vivo studies validated TET's effects on OS cells and signaling pathways.

Main Results:

  • TET significantly inhibited proliferation, migration, and invasion while promoting apoptosis in 143B and MG63 OS cells.
  • TET modulated multiple signaling pathways, including MAPK/Erk, PTEN/Akt, Juk, and Wnt.
  • In vivo experiments confirmed TET's regulatory effects on OS cells and associated signaling.

Conclusions:

  • TET demonstrates potent anticancer activity against human osteosarcoma by targeting multiple signaling pathways.
  • TET holds potential as a single agent or in combination therapy for osteosarcoma treatment.
Keywords:
Osteosarcomaanticancer effectcell growthmultiple signaling pathwaystetrandrine

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