Related Experiment Video
Updated: Oct 21, 2025
A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation
Published on: January 9, 2019
pepsickle rapidly and accurately predicts proteasomal cleavage sites for improved neoantigen identification
Benjamin R Weeder1,2, Mary A Wood3, Ellysia Li4
1Computational Biology Program, Oregon Health & Science University, Portland, OR 97239, USA.
Motivation:
Proteasomal cleavage is a key component in protein turnover, as well as antigen processing and presentation. Although tools for proteasomal cleavage prediction are available, they vary widely in their performance, options and availability.
Results:
Herein, we present pepsickle, an open-source tool for proteasomal cleavage prediction with better in vivo prediction performance (area under the curve) and computational speed than current models available in the field and with the ability to predict sites based on both constitutive and immunoproteasome profiles. Post hoc filtering of predicted patient neoepitopes using pepsickle significantly enriches for immune-responsive epitopes and may improve current epitope prediction and vaccine development pipelines.
Availability And Implementation:
pepsickle is open source and available at https://github.com/pdxgx/pepsickle.
Supplementary Information:
Supplementary data are available at Bioinformatics online.
