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A novel method for ex-vivo latex angiography of the mouse brain
Keyan Peterson1, Stephanie Coffman1, Stacey Wolfe1
1Department of Neurological Surgery, Wake Forest University School of Medicine, 1 Medical Center Blvd, Winston-Salem, NC 27157, USA.
Introduction:
Latex perfusion is an effective tool to study cerebrovascular pathology in the animal brain. It provides, low-cost, high fidelity anatomical information on ex-vivo analysis, and can be utilized to study multiple, states. However, current methods of latex casting and tissue-clearance do not allow for immunohistochemical analysis following sample processing. This results in experiments that require increased numbers of animals to attain adequate data.
New Method:
In this paper, we present a modified latex perfusion and tissue processing protocol for ex-vivo analysis, of the cerebral vasculature. The method consists of injection of the arterial tree with liquid latex, followed by tissue clearance with a scale solution.
Results:
Our results demonstrate effective and reliable perfusion of the murine cerebrovascular tree, rendering the arterial morphology of the brain in high detail, while allowing for post-perfusion, immunohistochemistry of the sample.
Comparison With Existing Method:
Our technique bypasses the limitations of previous latex angiography protocols by allowing for postperfusion, pathologic analysis of casted cerebrovascular tissue.
Conclusion:
This protocol provides a reliable, low-cost, method of cerebrovascular perfusion that reduces the number of animals required to generate robust data from latex-casted brain tissue.
