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OsBC1L1 and OsBC1L8 function in stomatal development in rice.

Zhengzheng Li1, Pengyue Sun1, Peng Sun1

  • 1State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China.

Biochemical and Biophysical Research Communications
|September 3, 2021
PubMed
Summary

Rice OsBC1L1 and OsBC1L8 genes are crucial for proper stomatal development. Their combined absence leads to abnormal stomatal production and defective structures, impacting plant gas exchange.

Keywords:
Cell divisionOsBC1L1OsBC1L8RiceStomatal development

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Area of Science:

  • Plant Biology
  • Developmental Genetics
  • Molecular Plant Science

Background:

  • Stomata regulate plant gas exchange and transpiration.
  • Grass stomata possess a unique morphology with dumbbell-shaped guard cells and subsidiary cells.
  • Understanding the genetic regulation of stomatal development is key to improving crop resilience.

Purpose of the Study:

  • To investigate the roles of OsBC1L1 and OsBC1L8 in rice stomatal development.
  • To elucidate the molecular mechanisms underlying stomatal patterning and cell division in grasses.

Main Methods:

  • Generated knockout mutants for OsBC1L1 and OsBC1L8 in rice.
  • Created a double knockout mutant (osbc1l1 osbc1l8) and OsBC1L1 overexpression lines.
  • Analyzed stomatal morphology, development, and gene expression patterns.
  • Utilized cell biology techniques to determine protein localization.

Main Results:

  • Single knockouts of OsBC1L1 or OsBC1L8 showed no defects.
  • The double knockout mutant (osbc1l1 osbc1l8) exhibited excessive stomatal production and clustering.
  • Abnormal stomatal patterning and defective stomata with single subsidiary cells were observed in the double mutant.
  • Expression of key stomatal development genes (OsSPCH2, OsFAMA) was downregulated in osbc1l1 osbc1l8.
  • OsBC1L1 and OsBC1L8 localize to the cell plate and plasma membrane during cell division.

Conclusions:

  • OsBC1L1 and OsBC1L8 are essential for regulating rice stomatal complex development.
  • These genes likely function in cell division processes critical for proper stomatal formation.
  • Disrupting OsBC1L1 and OsBC1L8 function leads to significant defects in stomatal patterning and number.