Modification of hydrochar increased the capacity to promote anaerobic digestion

Hydrochar has been demonstrated to increase methane production rate during anaerobic digestion (AD) of organic wastes/wastewater by facilitating direct interspecies electron transfer (DIET). The present study compared the hydrochars prepared at different conditions (260 °C-1 h, 260 °C-8 h, 320 °C-1 h and 320 °C-8 h) on AD of glucose. Hydrochar prepared at lower temperature and residence time (260 °C-1 h) resulted in the highest methane production rate, which was 237% higher of control experiment without hydrochar. Modification of hydrochar (260 °C-1 h) by ball-milling further increased the capacity to increase methane production rate. Hydrothermal liquefaction (HTL) conditions affected the surface oxygen-containing functional groups that related with DIET, and hydrochar (260 °C-1 h) had higher peaks relating with C-O and O-H functional groups. Ball-milling enhanced the formation of such groups. Microbial analysis showed hydrochar (260 °C-1 h) by ball-milling resulted in the formation of different microbial communities as compared with control experiments, and Azospira and Methanosarcina were enriched, which might be involved in DIET.