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Updated: Jul 28, 2026

Light-Controlled Fermentations for Microbial Chemical and Protein Production
Published on: March 22, 2022
Progressive transitional studies of engineered Synechococcus from laboratory to outdoor pilot-scale cultivation for
Kaustubh R Sawant1, Prashant Savvashe2, Divyani Pal1
1DBT-ICT Centre for Energy Biosciences, Institute of Chemical Technology, Matunga, Mumbai, 400019, India.
Abstract:
Cyanobacterial research is impeded by the substantial discrepancies between laboratory studies and outdoor performances, despite successful demonstrations of genetically engineered strains for array of compounds. Therefore, evaluation of adaptive responses is necessary to achieve outdoor scale-up cultivation of cyanobacteria. Under current study, cyanobacterium Synechococcus elongatusPCC7942 engineered for ethylene biosynthesis, was gradually acclimatised, ensuring sustained and progressive transition from laboratory to outdoor conditions. Bubble size of 4.9 ± 0.2 mm and air-flow rate of 0.05 vvm in BG11 supplemented with 5 g/L bicarbonate giving mass transfer coefficient (KLa) of 10.48 h-1 yielded highest specific growth rate (0.24 h-1) with the transformants. At the 100 L photobioreactor scale, ethylene productivity of 1.5 mL.L-1.h-1 was achieved. A comprehensive investigation on photosynthetic responses of the transformants adapted to the outdoor conditions exhibited interesting photosynthetic electron transport regulations, involving antenna density modulation in response to diurnal and dynamic light transitions, indicating successful transition.
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