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Rapid gas-chromatographic assay of bupivacaine in plasma
Y Le Normand1, A Athouel, Y Blanloeil
1Département de Pharmacologie, Faculté de Médecine, Centre Hospitalier Universitaire, Hôpital Laennec, Nantes, France.
Fundamental & Clinical Pharmacology
|January 1, 1987
Abstract:
A method for estimating bupivacaine concentration in human plasma by capillary gas-chromatography using solid injection and nitrogen-specific detection is described. Etidocaine, another anilidetype local anesthetic was used as internal standard and added to the sample before single-step extraction with diethylether. This method demonstrates high sensitivity (6 ng/ml plasma) and combines selectivity, rapidity, and simplicity. Results of this procedure correlate well with those obtained by an HPLC method.