Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Disruption of the brain-spleen axis impairs monocyte-microglia communication and accelerates disease progression in a mouse model of amyloidosis.

Nature communications·2026

Same author

Multiparametric whole-body magnetic resonance imaging in breast cancer management: A prospective study.

Tumori·2026

Same author

Parsing the functions of immediate-early proteins in the lytic-latent balance of HCMV infection.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

InSituPREP enables 3D single-cell mapping of interaction-associated gene programs in the breast cancer tumor microenvironment.

Nucleic acids research·2026

Same author

Evolving landscape of targeted therapies in early phase clinical trials.

European journal of cancer (Oxford, England : 1990)·2026

Same author

Heterogeneity and plasticity of cancer-associated fibroblasts.

Nature cancer·2026

Same journal

ClairS: a deep-learning method for long-read tumor-normal pair somatic small variant calling.

Nature methods·2026

Same journal

RNAbpFlow: base pair-augmented SE(3) flow matching for conditional RNA 3D structure generation.

Nature methods·2026

Same journal

Spatio-DARLIN enables robust and efficient in situ lineage tracing in mice at single-cell resolution.

Nature methods·2026

Same journal

EasyGrid: a versatile platform for automated cryo-EM sample preparation and quality control.

Nature methods·2026

Same journal

Cloud-based microscope enables live neuroimaging for 24 h and beyond with worldwide access.

Nature methods·2026

Same journal

Deep molecular profiling in three dimensions.

Nature methods·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Oct 21, 2025

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

Multiplexed profiling facilitates robust m6A quantification at site, gene and sample resolution.

David Dierks^1,2, Miguel Angel Garcia-Campos¹, Anna Uzonyi^1,3

¹Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.

|September 4, 2021

Summary

This summary is machine-generated.

N6-methyladenosine (m6A) sequencing is now more efficient with m6A-seq2. This method quantifies m6A modifications across sites, genes, and samples, revealing m6A as a key factor in RNA stability.

More Related Videos

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

Related Experiment Videos

Last Updated: Oct 21, 2025

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

Area of Science:

Molecular Biology
Epigenetics
RNA Biology

Background:

N6-methyladenosine (m6A) is the most abundant mRNA modification in mammals.
Quantifying m6A at site, gene, and sample levels is crucial for understanding its function.
Existing methods have limitations in throughput, variability, and scope.

Purpose of the Study:

To develop a high-throughput, cost-effective method for quantifying m6A modifications.
To enable simultaneous quantification of m6A at multiple biological levels.
To establish a computational framework for gene-level m6A analysis.

Main Methods:

Development of m6A-seq2, a technique using multiplexed m6A-immunoprecipitation of barcoded and pooled samples.
Implementation of a computational approach for gene-level m6A quantitation.
Validation of m6A-seq2's ability to provide sample-level relative quantitations.

Main Results:

m6A-seq2 significantly increases throughput and reduces technical variability, input material requirements, and cost.
The method provides orthogonal sample-level relative quantitations of m6A.
Gene-level m6A quantitation explains approximately 30% of RNA half-life variability in mouse embryonic stem cells.

Conclusions:

m6A-seq2 offers a comprehensive experimental and analytical framework for m6A research.
m6A is identified as a major determinant of RNA stability.
The developed method facilitates deeper dissection of m6A-mediated gene regulation.