Related Experiment Video

Updated: Oct 21, 2025

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
04:25

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

10.2K

The circular RNA circCRIM1 inhibits osteosarcoma progression through sponging miR-513

Pengfei Wu1, Yinghui Kong2, Zhitang Dai1

  • 1Department of Orthopaedics, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, No.1, Huanghe West Road, Huaiyin District, Huaian, 223300, Jiangsu, China.

Mammalian Genome : Official Journal of the International Mammalian Genome Society
|September 4, 2021
PubMed

Insights

Circular RNA CRIM1 (circCRIM1) is downregulated in osteosarcoma (OS), inhibiting tumor growth and metastasis. CircCRIM1 acts as a competing endogenous RNA (ceRNA) for miR-513, suggesting it as a potential therapeutic target for OS.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are implicated in tumor progression.
  • The role of circCRIM1 in osteosarcoma (OS) remains unclear.

Purpose of the Study:

  • To investigate the function and mechanism of circCRIM1 in osteosarcoma.
  • To explore circCRIM1 as a potential therapeutic target for OS.

Main Methods:

  • Quantification of circCRIM1 expression in OS tissues and cell lines using real-time PCR.
  • Assessment of OS cell proliferation, migration, and invasion using MTT, EDU, transwell migration, and invasion assays.
  • Mechanistic investigation of the circCRIM1/miR-513 axis.

Main Results:

  • circCRIM1 expression was significantly decreased in OS tissues and cell lines.
  • Lower circCRIM1 levels correlated with lymph node metastasis, advanced FIGO stage, and reduced overall survival.
  • circCRIM1 overexpression suppressed OS cell proliferation, migration, and invasion.
  • circCRIM1 functions as a ceRNA for miR-513, inhibiting OS progression.

Conclusions:

  • The circCRIM1/miR-513 pathway plays a critical role in OS cell proliferation, migration, and invasion.
  • circCRIM1 demonstrates potential as a therapeutic target for osteosarcoma treatment.

More Related Videos

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate07:31

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate

Published on: May 3, 2021

3.9K
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.7K

Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.5K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.3K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.0K
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.8K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.7K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.3K