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An indirect, quantifiable assay for cytophilic antibody
Journal of Immunological Methods
|January 1, 1978
Summary
A new method quantifies antibodies that bind to sheep red blood cells (SRBC) on macrophages. This indirect assay is faster and more reliable than visual methods for measuring antibody-macrophage interactions in vitro.
Area of Science:
- Immunology
- Cell Biology
Background:
- Antibodies cytophilic for macrophages play a crucial role in immune responses.
- Accurate in vitro measurement of these antibodies is essential for immunological research.
Purpose of the Study:
- To describe a novel, indirect, and quantifiable method for measuring anti-SRBC (sheep red blood cell) antibody that is cytophilic for macrophages.
Main Methods:
- Macrophages were incubated with serum dilutions, washed, and then incubated with 51Cr-labeled SRBC.
- Total counts per minute (cpm) were measured before and after washing to determine percentage binding.
- The assay was performed at 4 degrees C in 35 mm petri dishes.
Main Results:
- The assay provides quantifiable data on antibody binding to macrophages.
- Results showed consistency with visual determinations of SRBC count per macrophage.
- The method demonstrated reduced variability and increased speed and ease compared to visual assessment.
Conclusions:
- This indirect, quantifiable assay offers a reliable and efficient alternative for measuring macrophage-associated antibodies.
- The developed method enhances the study of antibody-mediated cellular interactions in vitro.