Quercetin Mitigates Red Blood Cell Membrane Bound Na⁺, K⁺-ATPase Transporter During Human Aging

Increasing interest has recently focused on determining whether quercetin may exert anti-aging properties or not? The objective of this study was determination of Na⁺, K⁺ -ATPase activity in quercetin-treated red blood cells during human aging. The study was carried out on human blood samples. The subjects were divided into different age groups, young, middle, and old. The effects of quercetin were evaluated by determining Na⁺, K⁺ -ATPase activity by co-incubating the red blood cells in presence of quercetin (10^-6 M to 10^-3 M final concentration). Quercetin causes 15% increase in Na⁺, K⁺ -ATPase activity at 10^-4 M and 17% at 10^-3 M as compared to the young control age group. The effect was insignificant at 10^-5 M (7%) and 10^-6 M (5%) in the young age group. Quercetin showed significant increase at 10^-6 M to 10^-3 M in Na⁺, K⁺ -ATPase activity as compared to the middle control age group. A significant increase in Na⁺, K⁺ -ATPase activity was observed at all concentrations [10^-6 M (31%), 10^-5 M (39%), 10^-4 M (51%), and 10^-3 M (61%)] in elderly population. We believe that these findings will help in further research against oxidative stress in red blood cells.