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Evolutionary coupling range varies widely among enzymes depending on selection pressure.

Julian Echave1

  • 1Instituto de Ciencias Físicas, Escuela de Ciencia y Tecnología, Universidad Nacional de San Martín, San Martín, Buenos Aires, Argentina.

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Summary

Evolutionary coupling in enzymes is not always long-range, contrary to previous studies. Its variable range, from 2 to 20 Å, is driven by functional selection, not physical interactions.

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Area of Science:

  • Biophysics
  • Evolutionary Biology
  • Enzymology

Background:

  • Recent research suggests enzyme active sites impose long-range evolutionary constraints.
  • The physical basis for these long-range evolutionary couplings remains unclear.

Purpose of the Study:

  • To investigate the relationship between physical and evolutionary couplings in monomeric enzymes.
  • To determine the range of evolutionary coupling and its physical origins.

Main Methods:

  • Utilized a biophysical model of evolution.
  • Analyzed a diverse dataset of monomeric enzymes.

Main Results:

  • Evolutionary coupling range is not universally long-range, varying from 2 to 20 Å across enzymes.
  • Physical coupling is consistently short-range for all enzymes studied.
  • Evolutionary coupling range is dictated by functional selection pressure, not physical coupling range.

Conclusions:

  • The range of evolutionary coupling in enzymes is variable and influenced by functional demands.
  • Physical interactions do not solely determine the extent of evolutionary coupling.