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siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...

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Updated: Oct 21, 2025

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

A design approach for layer-by-layer surface-mediated siRNA delivery.

Jonathan J Chou¹, Adam G Berger², Sasan Jalili-Firoozinezhad³

¹Institute for Soldier Nanotechnologies, Massachusetts Institute of Technology, Cambridge, MA 02139, United States; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, United States; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, United States.

Acta Biomaterialia

|September 4, 2021

Summary

Optimizing layer-by-layer assembly conditions enhances short interfering RNA (siRNA) delivery from coatings. This study reveals how parameters like pH and concentration impact siRNA efficacy for therapeutic applications.

Keywords:

Delivery Design of experiment Layer-by-layer PBAE siRNA

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Last Updated: Oct 21, 2025

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

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Published on: January 15, 2015

Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes

Delivery of Therapeutic siRNA to the CNS Using Cationic and Anionic Liposomes

Published on: July 23, 2016

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Area of Science:

Biomaterials Science
Nanotechnology
Molecular Biology

Background:

Short interfering RNA (siRNA) therapeutics offer targeted gene silencing for various diseases.
Effective delivery systems are crucial for clinical siRNA utility.
Layer-by-layer (LbL) technology facilitates siRNA formulation in polyelectrolyte thin films for localized delivery.

Purpose of the Study:

To investigate the impact of LbL assembly parameters on siRNA-eluting film composition.
To determine how these parameters influence in vitro siRNA-mediated gene knockdown efficiency.
To develop optimized conditions for effective and efficient siRNA surface delivery.

Main Methods:

Utilized a fractional factorial design to vary LbL assembly parameters (pH, ionic strength, polymer/siRNA concentration).
Assembled poly(β-amino ester) (PBAE) and siRNA films on polyglactin 910 sutures.
Quantified PBAE and siRNA loading, weight ratios, and assessed in vitro gene knockdown.

Main Results:

Identified key LbL assembly parameters affecting film composition and siRNA loading.
Demonstrated that optimized conditions yielded significant in vitro gene knockdown (47%).
Developed a rationally designed formulation achieving high knockdown with fewer layers, reducing complexity and cost.

Conclusions:

LbL solution conditions critically influence the performance of surface-mediated siRNA delivery systems.
The study presents an adaptable methodology for optimizing siRNA delivery via LbL coatings.
Findings pave the way for improved siRNA therapeutics and delivery of other nucleic acids.