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Glucosamine to gold nanoparticles binding studied using Raman spectroscopy
Vlasta Mohaček-Grošev1, Sandro Brljafa2, Marko Škrabić3
1Center of Excellence for Advanced Materials and Sensing Devices, Research Unit New Functional Materials, Ruđer Bošković Institute, Bijeničkacesta 54, 10000 Zagreb, Croatia.
Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|September 4, 2021
Summary
This study used surface-enhanced Raman scattering (SERS) to confirm glucosamine binding to gold nanoparticles (AuNPs). The results help identify how amino acids interact with AuNPs in various solutions.
Area of Science:
- Nanomaterials Science
- Biophysical Chemistry
- Spectroscopy
Background:
- Colloidal gold nanoparticles (AuNPs) are widely used in biosensing.
- Glucosamine is a key biomolecule with potential for AuNP interaction.
- Understanding molecular binding to AuNPs is crucial for developing advanced sensors.
Purpose of the Study:
- To investigate the binding of glucosamine to gold nanoparticles (AuNPs) in aqueous solutions.
- To determine if the charged amino group of glucosamine interacts with AuNPs.
- To identify characteristic SERS signals indicative of glucosamine-AuNP binding.
Main Methods:
- Surface-enhanced Raman scattering (SERS) spectroscopy.
- Dynamic light scattering (DLS) for nanoparticle size analysis.
- UV-Vis spectroscopy.
- Transmission electron microscopy (TEM) for dried nanoparticle size.
- Density functional theory (DFT) calculations for vibrational analysis.
Main Results:
- SERS spectra revealed distinct vibrational bands associated with glucosamine binding to AuNPs, particularly at 1165, 1532, and 1586 cm⁻¹.
- Hydrodynamic diameter of AuNPs increased with glucosamine concentration, indicating aggregation or complex formation.
- Specific bands at 999 and 1075 cm⁻¹ in 1 mM glucosamine solution were attributed to interactions involving the C-O ring and C-NH₃⁺ groups.
- Anomer-specific bands for glucose were observed, but distinguishing alpha and beta glucosamine anomers on AuNPs proved complex.
Conclusions:
- Glucosamine does bind to gold nanoparticles, with evidence from SERS spectral changes.
- The study successfully identified key vibrational markers for glucosamine-AuNP interactions.
- These findings provide a basis for using SERS to study amino acid interactions with nanomaterials, aiding in the development of new analytical techniques.
