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Related Experiment Video

Updated: Oct 21, 2025

Author Spotlight: An Alternative Approach to Protein Quantification by Bradford Assay Using a Smartphone
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Quantifying Protein Concentrations Using Smartphone Colorimetry: A New Method for an Established Test.

Clifford T Gee1, Eric Kehoe2, William C K Pomerantz1

  • 1Department of Chemistry, University of Minnesota, Minneapolis, Minnesota 55455, United States.

Journal of Chemical Education
|September 6, 2021
PubMed
Summary
This summary is machine-generated.

This study demonstrates smartphone colorimetry for quantifying protein concentration using Bradford and biuret assays. This accessible method enables accurate protein measurement in educational laboratory settings.

Keywords:
Analytical ChemistryBiochemistryFirst-Year Undergraduate/GeneralHands-On Learning/ManipulativesHigh School/Introductory ChemistryLaboratory InstructionProteins/PeptidesQuantitative AnalysisUV–Vis Spectroscopy

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biotechnology

Background:

  • Proteins are fundamental to biological processes, driving interest in accurate quantification methods.
  • Colorimetric assays are established techniques for analyte concentration determination.
  • Previous research utilized hand-held cameras for colorimetric analysis of solutions.

Purpose of the Study:

  • To adapt and validate smartphone colorimetry for protein concentration quantification.
  • To assess the efficacy of the Bradford and biuret assays using smartphone-based colorimetry.
  • To establish an accessible laboratory experiment for protein quantification.

Main Methods:

  • Utilized smartphone colorimetry to analyze color changes in biochemical reactions.
  • Employed the Bradford assay for protein quantification.
  • Employed the biuret assay for protein quantification.
  • Developed an experimental setup suitable for educational purposes.

Main Results:

  • Successfully quantified protein concentration using smartphone colorimetry with both Bradford and biuret assays.
  • Demonstrated a color change (e.g., green to blue) correlating with protein concentration.
  • Validated the accuracy and ease of the smartphone-based method.

Conclusions:

  • Smartphone colorimetry provides a viable and accessible method for protein quantification.
  • The Bradford and biuret assays are effectively adaptable to smartphone colorimetry.
  • This technique offers a valuable tool for high school and college-level biochemistry laboratories.