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Updated: Oct 21, 2025

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Protective Effect of Genistein on Cyclin D1 Expression in Malignant Ocular Melanoma Cells

Yasmin Sani Aprilliantina1, Hera Dwi Novita1, Elfina G Sadono1

  • 1Department of Ophtalmology, Dr. Saiful Anwar General Hospital/Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia.

Medical Archives (Sarajevo, Bosnia and Herzegovina)
|September 6, 2021
PubMed
Abstract

Insights

Genistein affects cyclin D1 expression in malignant melanoma cells in a dose-dependent manner. Low to moderate doses increase expression, while high doses decrease it, suggesting potential therapeutic applications.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Ocular melanoma is a rare but aggressive malignancy affecting various eye tissues.
  • Uveal melanoma is the most common subtype, while conjunctival melanoma is exceptionally rare.
  • Understanding molecular mechanisms is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the impact of genistein on cyclin D1 expression in malignant melanoma cells.
  • To determine the dose-dependent effects of genistein on a key cell cycle regulator.

Main Methods:

  • Malignant melanoma cells (CRL1872) were treated with varying genistein concentrations (25 μM, 50 μM, 100 μM).
  • Cyclin D1 expression was quantified using immunofluorescence.
  • Confocal laser scanning microscopy was employed for precise cellular analysis.

Main Results:

  • Genistein at 25 μM and 50 μM significantly increased cyclin D1 expression (p < 0.05).
  • A significant decrease in cyclin D1 expression was observed at 100 μM genistein compared to controls (p < 0.05).

Conclusions:

  • Genistein exhibits a biphasic effect on cyclin D1 expression in malignant melanoma.
  • Appropriate dosing of genistein may represent a viable therapeutic strategy for malignant melanoma.
Keywords:
biphasic effectchemotherapyocular melanomasoybeans

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