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Related Experiment Video

Updated: Oct 21, 2025

Establishment of a Novel Ex Vivo Lung Perfusion System for Rat Lungs After Circulatory Death
09:14

Establishment of a Novel Ex Vivo Lung Perfusion System for Rat Lungs After Circulatory Death

Published on: October 18, 2024

648

Ex vivo lung perfusion: how we do it.

John Santosh Murala1, William Michael Whited1, Amit Banga2

  • 1Department of Cardiovascular and Thoracic Surgery, University of Texas Southwestern (UTSW) Medical Center, 5959 Harry Hines Blvd., 10th Floor, Suite HP10.110, Dallas, TX 75390 USA.

Indian Journal of Thoracic and Cardiovascular Surgery
|September 6, 2021
PubMed
Summary

Ex vivo lung perfusion (EVLP) enhances lung transplantation by enabling evaluation and repair of donor lungs, expanding the donor pool. This technique has increased lung transplant volumes by 12% annually since FDA approval.

Keywords:
Donor selectionEx vivo lung perfusionLung transplantation

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Area of Science:

  • Thoracic Surgery
  • Transplantation Medicine
  • Pulmonology

Background:

  • Lung transplantation is a vital treatment for end-stage lung disease.
  • Donor organ shortage, low lung utilization, and waitlist mortality remain significant challenges.
  • Ex vivo lung perfusion (EVLP) has emerged as a key innovation in recent years.

Purpose of the Study:

  • To detail the authors' experience with ex vivo lung perfusion (EVLP) in lung transplantation.
  • To describe the workflow, setup, operative techniques, and protocols of a multidisciplinary EVLP program.
  • To highlight the impact of EVLP on expanding the donor pool and increasing transplant volumes.

Main Methods:

  • Implementation of a multidisciplinary EVLP program involving surgeons, pulmonologists, and specialized nurses.
  • Detailed description of the EVLP workflow, including lung evaluation and potential reparative therapies.
  • Adherence to established protocols for EVLP procedures.

Main Results:

  • EVLP allows for prolonged evaluation of marginal donor lungs, increasing their suitability for transplantation.
  • Reparative therapies can be applied to lungs previously deemed unsuitable for transplant.
  • The EVLP program has been a valuable addition, contributing to an annual increase in lung transplant volume of 12% since FDA approval in 2019.

Conclusions:

  • Ex vivo lung perfusion (EVLP) is an effective strategy to overcome donor lung limitations.
  • EVLP expands the donor pool and facilitates lung transplantation for more patients.
  • The collaborative, multidisciplinary approach to EVLP is crucial for its success.