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Microarray Analysis Identifies Key Differentially Expressed Circular RNAs in Aged Mice With Postoperative Cognitive

Yu-Qing Wu1, Qiang Liu1, Hai-Bi Wang1

  • 1Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical University, Xuzhou, China.

Frontiers in Aging Neuroscience
|September 6, 2021
PubMed
Summary
This summary is machine-generated.

Circular RNAs (circRNAs) are implicated in postoperative cognitive dysfunction (POCD) in aged mice. This study identified 124 differentially expressed circRNAs in a POCD model, suggesting their role in neuroinflammation and cognitive decline.

Keywords:
ceRNA networkcircRNAsexpression profilemiRNAspostoperative cognitive dysfunction

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Postoperative cognitive dysfunction (POCD) is a frequent complication in elderly individuals.
  • Circular RNAs (circRNAs) are increasingly recognized for their potential role in neurodegenerative diseases.
  • The specific involvement of circRNAs in POCD in aged populations remains largely unexplored.

Purpose of the Study:

  • To investigate the expression profiles and potential roles of circRNAs in a mouse model of POCD.
  • To identify differentially expressed circRNAs and construct a competing endogenous RNA (ceRNA) network.
  • To elucidate the molecular mechanisms underlying circRNA involvement in POCD.

Main Methods:

  • circRNA microarray analysis to screen for differentially expressed circRNAs.
  • Quantitative real-time polymerase chain reaction (qRT-PCR) for validation.
  • Bioinformatic analyses including ceRNA network construction, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, and protein-protein interaction network analysis using STRING and Cytoscape.

Main Results:

  • Microarray analysis identified 124 differentially expressed circRNAs in the hippocampus of aged mice with POCD.
  • Upregulated and downregulated circRNAs were found to be involved in various signaling pathways relevant to neuroinflammation and cognitive function.
  • Hub genes, such as Egfr and Prkacb, were predicted to be regulated by ceRNA networks.

Conclusions:

  • circRNAs are dysregulated in the hippocampus of aged mice experiencing POCD.
  • These circRNAs, potentially through ceRNA networks, may play a significant role in the pathogenesis of POCD.
  • Further research into circRNA function could reveal novel therapeutic targets for preventing or treating POCD.