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Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
Published on: September 18, 2020
Genome-Wide DNA Methylation Pattern in Whole Blood Associated With Primary Intracerebral Hemorrhage
Yupeng Zhang1, Hongyu Long1, Sai Wang1
1Department of Neurology, Xiangya Hospital, Central South University, Changsha, China.
Insights
DNA methylation patterns differ significantly in patients with intracerebral hemorrhage (ICH). These epigenetic changes in peripheral blood may contribute to ICH development and warrant further investigation.
Area of Science:
- Epigenetics
- Genomics
- Neuroscience
Background:
- Primary intracerebral hemorrhage (ICH) is a major global health concern, causing significant morbidity and mortality.
- ICH is a complex disease influenced by genetic and environmental factors.
- DNA methylation is recognized for its role in the etiology of complex diseases.
Purpose of the Study:
- To investigate DNA methylation patterns in peripheral blood of patients with ICH.
- To identify differences in DNA methylation between ICH patients and healthy controls.
Main Methods:
- Utilized the Illumina Infinium Human Methylation 850k BeadChip array.
- Compared DNA methylation profiles in whole blood samples from 30 ICH patients and 34 healthy controls.
Main Results:
- Identified 1530 significantly differentially methylated sites between ICH patients and controls (p < 5.92E-08).
- Found 1377 hypermethylated and 153 hypomethylated sites in ICH patients.
- Observed enrichment of differentially methylated genes in inflammatory pathways.
Conclusions:
- Significant differences in DNA methylation patterns exist in the peripheral blood of ICH patients.
- These epigenetic alterations may play a role in the pathogenesis of intracerebral hemorrhage.
- Further research is warranted to explore the implications of these findings.
Abstract:
Primary intracerebral hemorrhage (ICH) is a significant cause of morbidity and mortality throughout the world. ICH is a multifactorial disease that emerges from interactions among multiple genetic and environmental factors. DNA methylation plays an important role in the etiology of complex traits and diseases. We used the Illumina Infinium Human Methylation 850k BeadChip to detect changes in DNA methylation in peripheral blood samples from patients with ICH and healthy controls to explore DNA methylation patterns in ICH. Here, we compared genomic DNA methylation patterns in whole blood from ICH patients (n = 30) and controls (n = 34). The ICH and control groups showed significantly different DNA methylation patterns at 1530 sites (p-value < 5.92E-08), with 1377 hypermethylated sites and 153 hypomethylated sites in ICH patients compared to the methylation status in healthy controls. A total of 371 hypermethylated sites and 35 hypomethylated sites were in promoters, while 738 hypermethylated sites and 67 hypomethylated sites were in coding regions. Furthermore, the differentially methylated genes between ICH patients and controls were largely related to inflammatory pathways. Abnormalities in the DNA methylation pattern identified in the peripheral blood of ICH patients may play an important role in the development of ICH and warranted further investigation.
