Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of thalidomide on sporadic central nervous system arteriovenous malformations.

Science bulletin·2026
Same author

Influence of Luteolin on Physicochemical Characteristics, Structural Changes and Functional Properties of Casein Fermentation System.

Foods (Basel, Switzerland)·2026
Same author

SPAID: a comprehensive database for disease-specific autoantigens in autoimmune disorders.

Advanced biotechnology·2026
Same author

Noninvasive Volume Measurement of Cerebral Arteriovenous Malformation Nidus: Silent MRA Compared with TOF-MRA.

AJNR. American journal of neuroradiology·2026
Same author

How to make big data accessible to plant biologists and beyond: Ten years of lessons from TBtools.

Molecular plant·2026
Same author

Modulating C─F Bonds of Fluorinated Graphite via Compounding Graphene Oxide for High-Energy/Power Lithium Batteries.

Small (Weinheim an der Bergstrasse, Germany)·2026

Related Experiment Video

Updated: Oct 21, 2025

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates
13:49

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates

Published on: December 6, 2017

11.6K

RAPID: A Rep-Seq Dataset Analysis Platform With an Integrated Antibody Database.

Yanfang Zhang1,2,3,4,5, Tianjian Chen6, Huikun Zeng1,2,3,4,5

  • 1State Key Laboratory of Organ Failure Research, National Clinical Research, Center for Kidney Disease, Division of Nephrology, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Frontiers in Immunology
|September 6, 2021
PubMed
Summary
This summary is machine-generated.

Researchers can now analyze antibody repertoires with RAPID, a new web tool. It integrates vast antibody data and Rep-seq datasets, aiding adaptive immunity studies and antibody discovery.

Keywords:
Rep-Seqantibody annotationantibody databasecomparative analysispublic clone

More Related Videos

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
08:51

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Published on: March 15, 2019

12.6K
Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein
08:04

Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein

Published on: December 20, 2017

7.1K

Related Experiment Videos

Last Updated: Oct 21, 2025

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates
13:49

Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates

Published on: December 6, 2017

11.6K
Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
08:51

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Published on: March 15, 2019

12.6K
Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein
08:04

Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein

Published on: December 20, 2017

7.1K

Area of Science:

  • Immunology
  • Bioinformatics
  • Computational Biology

Background:

  • The antibody repertoire is crucial for adaptive immunity, reflecting immune history and status.
  • Understanding antibody repertoires advances humoral immunity knowledge, antibody discovery, and disease diagnosis/treatment.
  • Existing tools lack integration of large Rep-seq data with functional antibody knowledge.

Purpose of the Study:

  • To develop an integrated platform for analyzing antibody repertoires using Rep-seq data.
  • To provide a user-friendly, web-based tool for researchers to process and analyze antibody repertoire datasets.
  • To facilitate the elucidation of the diverse antibody repertoire by combining big data and prior knowledge.

Main Methods:

  • Development of the Rep-seq dataset Analysis Platform with an Integrated antibody Database (RAPID).
  • Integration of 521 WHO-recognized therapeutic antibodies, 88,059 antigen/disease-specific antibodies, and 306 million clones from 2,449 human IGH Rep-seq datasets.
  • Implementation of a standardized Rep-seq dataset analysis pipeline with automatic clone annotation and query functions.

Main Results:

  • RAPID consolidates a comprehensive database of therapeutic, antigen-specific, and disease-associated antibodies.
  • The platform processed 2,449 human IGH Rep-seq datasets from individuals with 29 different health conditions.
  • Users can upload, analyze, and compare their Rep-seq datasets, querying antibodies by sequence or keywords.

Conclusions:

  • RAPID offers a powerful, free, web-based solution for analyzing antibody repertoires.
  • The integrated database and analysis pipeline enhance the study of adaptive immunity.
  • RAPID is expected to significantly benefit researchers in antibody discovery and understanding immune responses.