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QTL Mapping and Validation for Kernel Area and Circumference in Common Wheat via High-Density SNP-Based Genotyping
Tianheng Ren1,2, Tao Fan1,2, Shulin Chen1,2
1College of Agronomy, Sichuan Agricultural University, Chengdu, China.
Frontiers in Plant Science
|September 6, 2021
Summary
Researchers identified novel quantitative trait loci (QTLs) for wheat kernel area (KA) and kernel circumference (KC). These findings enhance understanding of kernel size genetics and aid marker-assisted selection for improved wheat yield traits.
Area of Science:
- Plant genetics
- Agricultural science
- Wheat breeding
Background:
- Thousand kernel weight (TKW) is crucial for wheat yield, with kernel size being a key determinant.
- While many loci for wheat kernel size exist, those for kernel area (KA) and kernel circumference (KC) are less understood.
Purpose of the Study:
- To map quantitative trait loci (QTLs) controlling kernel area (KA) and kernel circumference (KC) in wheat.
- To identify novel major and stable QTLs for KA and KC.
- To develop molecular markers for marker-assisted selection.
Main Methods:
- Utilized a recombinant inbred lines (RIL) population of 371 lines genotyped with the Wheat55K SNP array.
- Employed inclusive composite interval mapping to identify QTLs across multiple environments.
- Developed Kompetitive allele-specific PCR (KASP) markers linked to significant QTLs.
Main Results:
- Mapped a total of 54 and 44 QTLs for KA and KC, respectively.
- Identified 22 major QTLs, including novel and stable QTLs QKc.sau-6A.1 and QKa.sau-6A.2, explaining significant phenotypic variation.
- Developed two KASP markers (KASP-AX-109894590 and KASP-AX-109380327) tightly linked to these novel QTLs.
Conclusions:
- The study reveals the genetic basis of kernel area and circumference in wheat.
- Novel QTLs and developed KASP markers provide valuable tools for marker-assisted selection to improve kernel size traits.
- Lays the groundwork for fine mapping and cloning of genes controlling stable QTLs for kernel size.
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