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[Spontaneous bacteriophage induction in Bacillus thuringiensis]
Mikrobiologiia
|September 1, 1987
Summary
Temperate bacteriophage production in Bacillus thuringiensis was induced by germinating spores. Lysis occurred in some cells, leading to lytic cycles within the growing culture before the exponential phase concluded.
Area of Science:
- Microbiology
- Molecular Biology
- Bacteriology
Context:
- Investigating temperate bacteriophage production in Bacillus thuringiensis (Bt) lysogenic strains.
- Utilizing batch cultivation methods for controlled study conditions.
- Employing IPM-1148 as an indicator culture for precise phage titration.
Purpose:
- To elucidate the dynamics of temperate bacteriophage production during Bacillus thuringiensis batch cultivation.
- To determine the conditions and timing of phage induction and lytic cycle initiation.
- To quantify phage titres and their correlation with bacterial growth phases.
Summary:
- Temperate bacteriophage production was observed in three Bacillus thuringiensis lysogenic strains during batch cultivation.
- Phage growth was induced upon germination of thermoactivated spores, with some cells undergoing lysis without division.
- Subsequent lytic cycles occurred in the actively growing bacterial culture, with phage titres peaking before the end of the exponential growth phase.
Impact:
- Provides insights into the life cycle and replication strategies of temperate bacteriophages in Bacillus thuringiensis.
- Contributes to understanding phage-bacterial interactions in microbial ecosystems.
- Informs potential applications of bacteriophages in biocontrol or genetic engineering involving Bacillus thuringiensis.