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Synthetic Evaluation of MicroRNA-1-3p Expression in Head and Neck Squamous Cell Carcinoma Based on Microarray Chips
Yubing Chen1, Mingjiang Liu1, Hu Jin1
1Division of Hepatobiliary Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
Background:
MicroRNA-1-3p (miR-1-3p) exerts significant regulation in various tumor cells, but its molecular mechanisms in head and neck squamous cell carcinoma (HNSCC) are still ill defined. This study is aimed at detecting the expression of miR-1-3p in HNSCC and at determining its significant regulatory pathways.
Methods:
Data were obtained from the Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO), Oncomine, ArrayExpress, Sequence Read Archive (SRA) databases, and additional literature. Expression values of miR-1-3p in HNSCC were analyzed comprehensively. The R language software was employed to screen differentially expressed genes, and bioinformatics assessment was performed. One sequence dataset (HNSCC: n = 484; noncancer: n = 44) and 18 chip datasets (HNSCC: n = 656; noncancer: n = 199) were obtained.
Results:
The expression of miR-1-3p in HNSCC was visibly decreased in compare with noncancerous tissues. There were distinct differences in tumor state (P = 0.0417), pathological stage (P = 0.0058), and T stage (P = 0.0044). Comprehensive analysis of sequence and chip data also indicated that miR-1-3p was lowly expressed in HNSCC. The diagnostic performance of miR-1-3p in HNSCC is reflected in the sensitivity and specificity of the collection, etc. Bioinformatics analysis showed the possible biological process, cellular component, molecular function, and KEGG pathways of miR-1-3p in HNSCC. And ITGB4 was a possible target of miR-1-3p.
Conclusions:
miR-1-3p's low expression may facilitate tumorigenesis and evolution in HNSCC through signaling pathways. ITGB4 may be a key gene in targeting pathways but still needs verification through in vitro experiments.
Insights
MicroRNA-1-3p (miR-1-3p) is significantly downregulated in head and neck squamous cell carcinoma (HNSCC), suggesting its role in tumor development. Integrin beta 4 (ITGB4) may be a key target gene.
Area of Science:
- Oncology
- Molecular Biology
- Genomics
Background:
- MicroRNA-1-3p (miR-1-3p) plays a role in tumor cells, but its function in head and neck squamous cell carcinoma (HNSCC) is not well understood.
- This study investigates the expression and regulatory pathways of miR-1-3p in HNSCC.
Purpose of the Study:
- To determine the expression levels of miR-1-3p in HNSCC tissues compared to non-cancerous tissues.
- To identify the molecular mechanisms and signaling pathways regulated by miR-1-3p in HNSCC.
Main Methods:
- Utilized data from TCGA, GEO, Oncomine, ArrayExpress, and SRA databases.
- Performed comprehensive analysis of miR-1-3p expression in HNSCC using sequence and chip datasets.
- Employed R language for differential gene expression screening and bioinformatics analysis.
Main Results:
- miR-1-3p expression was significantly decreased in HNSCC tissues.
- Low miR-1-3p expression correlated with tumor state, pathological stage, and T stage.
- Bioinformatics analysis identified potential biological processes, cellular components, molecular functions, and KEGG pathways associated with miR-1-3p. Integrin beta 4 (ITGB4) was identified as a potential target.
Conclusions:
- Downregulation of miR-1-3p may promote HNSCC tumorigenesis and progression via specific signaling pathways.
- ITGB4 is a potential key gene in miR-1-3p targeting pathways, requiring further in vitro validation.
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