Diabetic Bone Marrow Cell Injection Accelerated Acute Pancreatitis Progression.

Area of Science:

• Gastroenterology and Hepatology
• Endocrinology and Metabolism
• Immunology

Background:

• Acute pancreatitis (AP) is a significant cause of hospitalization, with a subset progressing to severe forms.
• Diabetes mellitus is an established independent risk factor for AP incidence and severity.
• The precise mechanisms by which diabetes influences AP progression remain incompletely understood.

Purpose of the Study:

• To investigate the role of diabetic bone marrow cells (BMC) in the progression of acute pancreatitis.
• To elucidate the impact of diabetic BMC on inflammation and acinar cell injury during AP.

Main Methods:

• Acute pancreatitis was induced in wild-type mice using caerulein injections.
• Bone marrow cells were isolated from both wild-type and diabetic (db/db) mice.
• These BMC were transplanted into recipient mice, followed by AP induction and subsequent analysis of serum markers, histology, and cytokine production.

Main Results:

• Mice receiving diabetic BMC exhibited significantly elevated serum lipase activity and overall pancreatitis severity scores compared to controls and wild-type BMC recipients.
• Histological examination revealed increased acinar cell necrosis and vacuolization in mice treated with diabetic BMC.
• Diabetic BMC produced higher levels of pro-inflammatory cytokines, including IL-6, GM-CSF, and IL-10, and attracted more myeloperoxidase-positive cells.

Conclusions:

• Bone marrow cells from diabetic mice exacerbate acute pancreatitis.
• Diabetic BMC enhance the inflammatory response and acinar cell damage, contributing to AP progression.
• These findings underscore the detrimental role of diabetes-associated BMC in AP pathogenesis.