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Related Experiment Video

Updated: Oct 21, 2025

Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis
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KLF4 transactivates TRIM29 expression and modulates keratin network.

Runqing Huang1,2,3, Yang Fu1,2, Yanhong Deng1,2,3

  • 1Department of Medical Oncology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Biochemistry and Biophysics Reports
|September 6, 2021
PubMed
Summary
This summary is machine-generated.

Krüppel-like factor 4 (KLF4) activates TRIM29 gene expression and promotes cell migration. KLF4

Keywords:
KLF4KeratinMigrationTRIM29

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Gene Regulation

Background:

  • Krüppel-like factor 4 (KLF4) is a transcription factor involved in cell proliferation and differentiation.
  • The role of KLF4 in regulating TRIM29 expression and its impact on the keratin network are not fully understood.

Purpose of the Study:

  • To investigate the regulatory relationship between KLF4 and TRIM29.
  • To elucidate the functional impact of KLF4 on cell migration and keratin phosphorylation.

Main Methods:

  • Chromatin immunoprecipitation assays to confirm KLF4 binding to the TRIM29 promoter.
  • Western blotting to assess TRIM29 expression and keratin phosphorylation levels.
  • Cell migration assays and gene knockdown experiments.

Main Results:

  • KLF4 directly binds to the TRIM29 promoter and transactivates its transcription.
  • Sumoylation at lysine 278 is not essential for KLF4-mediated TRIM29 transactivation.
  • Overexpression of KLF4 enhances cell migration, an effect dependent on TRIM29.
  • KLF4 overexpression reduces keratin 8 phosphorylation at amino acid 432.

Conclusions:

  • KLF4 is a key regulator of TRIM29 expression.
  • KLF4 influences cell migration and keratin network organization through TRIM29 modulation.