[Microfluidic strategies for separation and analysis of circulating exosomes]

Exosomes are membrane-bound nanovesicles that are secreted by most types of cells and contain a range of biologically important molecules, including lipids, proteins, ribonucleic acids, etc. Emerging evidences show that exosomes can affect cells' physiological status by transmitting molecular messages among cells. As such, exosomes are involved in various pathological processes. Studying exosomes is of great importance for understanding their biological functions and relevance to disease diagnosis. However, it is difficult to separate and analyze exosomes due to their small size, and because their density is similar to that of bodily fluids. Traditional methods, including ultracentrifugation and ultrafiltration are time-consuming and require expensive equipment. Other methods for exosome separation, including immunoaffinity-based methods, are expensive and rely heavily on specific antibodies. Precipitation-based methods do not yield acceptable purity for downstream analysis, due to polymer contamination. Thus, urgent demand exists for a portable, simple, affordable method for exosome separation. Microfluidic chip technology offers a potential platform for separation and detection of exosomes, with several remarkable characteristics, including low sample consumption, high throughput, and easy integration. This paper provides an overview of current microfluidic strategies for separation and analysis of circulating exosomes. In our introduction to exosome separation, we divide existing separation methods into two categories. Category one is based on exosome physical properties, and includes membrane filtration, nano-column array sorting, and physical isolation. The other is immune capture, which is based on biochemical characteristics of exosomes, and includes fixed base immune capture and unfixed base immune capture. In our introduction to exosome analyses, some commonly used methods, including western blotting, scanning electron microscopy, and flow cytometry are briefly described. Some new systems, which combine microfluidic technology with fluorescence, electrochemical sensing, surface plasmon resonance, or other multimodal analysis methods for integrated detection of exosomes are then described in detail. Finally, the challenges faced by microfluidic technology in improving exosome purity and making systems more portable are analyzed. Prospects for application of microfluidic chips in this area are also discussed. With the rapid development of micro/nano-manufacturing, new materials, and information technology, microfluidic exosome separation and analysis systems will become smaller, more integrated, and more automated. Microfluidic chip technology will play important roles in exosome separation, biochemical detection, and mechanism analysis.