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Updated: Oct 21, 2025
FISH for Pre-implantation Genetic Diagnosis
Published on: February 23, 2011
[Application of mapping allele with resolved carrier status technique for preimplantation genetic testing in carriers
Qiuxiang Huang1, Yun Liu, Chunli Lin
1Department of Obstetrics & Gynecology, Center of Reproductive Medicine, Fuzong Clinical College of Fujian Medical University (900th Hospital of the Joint Logistics Team)/ Dongfang Hospital Affiliated to Xiamen University (900th Hospital of the Joint Logistics Team), Fuzhou, Fujian 350025, China. liuyunfj@126.com; xiaonvwang@163.com.
Objective:
To assess the application value of mapping allele with resolved carrier status (MaReCs) technique for preimplantation genetic testing (PGT).
Methods:
The characteristics of MaReCs for PGT and outcome of patients were retrospectively analyzed.
Results:
Compared with those who could not use the technique, carriers who have used the MaReCs technique were younger, had significantly higher level of anti-Mullerian hormone, more antral follicles, occytes, mature occytes, biopsied embryos and euploid embryos, and lower risks for de novo chromosomal abnormality (P<0.05). It was necessary for couples with fewer oocytes, mature oocytes and balstocyst to preserve discarded embryos to facilitate the test. Carriers who have used the MaReCs technique had higher clinical pregnancy rate and abortion rate compared with those undergoing routine PGT, albeit no significant difference was found between the two groups (P> 0.05). Carriers undergoing MaReCs test could preferentially select embryos with normal chromosome structures for the transfer.
Conclusion:
Application of MaReCs has a prerequisite for having a minimum number of occytes and biopsied embryos and using discarded embryos sometimes. MaReCs is efficient for the detection of carrier status of embryos and attaining higher rate of pregnancy and live birth, which can significantly improve the outcome for couples carrying chromosomal translocations.
