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Published on: September 22, 2017
A protocol for single-source dual-pulse stimulated emission depletion setup with Bessel modulation
Xinzhu Xu1,2, Kun Zhao1,2, Wei Ren1
1Department of Biomedical Engineering, College of Future Technology, Peking University, Beijing, People's Republic of China.
Abstract:
STimulated Emission Depletion (STED) microscopy attains super-resolution in biological imaging beyond the diffraction limit. Here, we give a concise protocol to construct a dual-pulse STED setup with one super-continuum laser. Moreover, a flexible and dismountable Bessel modulation module is introduced for potential 2D-stack STED imaging. Experiments and notices are introduced in detail, with discussion on some important check-points for STED, such as detector saturation. Finally, the results validate the system working.
