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Isolation of Adipogenic and Fibro-Inflammatory Stromal Cell Subpopulations from Murine Intra-Abdominal Adipose Depots

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Published on: August 16, 2020

Brown adipose tissue monocytes support tissue expansion.

Alexandre Gallerand¹, Marion I Stunault¹, Johanna Merlin¹

¹Université Côte d'Azur, INSERM, C3M, Nice, France.

Nature Communications

|September 7, 2021

Summary

Monocyte recruitment is crucial for brown adipose tissue (BAT) expansion and remodeling. Depleting monocytes prevents BAT growth, highlighting their essential role in tissue function.

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Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

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Published on: February 3, 2023

Area of Science:

Immunology
Metabolic Research
Cell Biology

Background:

Monocytes are key immune cells involved in inflammation.
Understanding monocyte dynamics in tissues like brown adipose tissue (BAT) may offer therapeutic targets.
Brown adipose tissue plays a role in energy expenditure and metabolic health.

Purpose of the Study:

To characterize monocyte and macrophage populations within BAT.
To investigate the impact of monocytes on BAT function during tissue remodeling.
To explore the role of monocyte recruitment in BAT expansion.

Main Methods:

Single-cell RNA sequencing of BAT immune cells.
Fate-mapping experiments to track macrophage origins.
Genetic models of BAT expansion.
Positron emission tomography (PET) using a CCR2-binding radiotracer.
Monocyte depletion studies.

Main Results:

Single-cell RNA sequencing revealed diverse monocyte and macrophage populations in BAT.
Fate-mapping confirmed continuous monocyte replenishment of the BAT macrophage pool.
Monocyte numbers were selectively increased during BAT expansion, confirmed by PET imaging.
Blood monocyte counts decreased during BAT expansion, without affecting bone marrow hematopoiesis.
Monocyte depletion inhibited BAT expansion and altered tissue architecture.
Podoplanin engagement was essential for BAT expansion.

Conclusions:

Monocyte recruitment is a critical driver of brown adipose tissue expansion and remodeling.
The study redefines the immune cell landscape within BAT, emphasizing monocyte contributions.
Targeting monocyte recruitment presents a potential therapeutic strategy for modulating BAT function.