Related Experiment Video
Updated: Oct 21, 2025
10:09
Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes
Published on: September 13, 2022
2.2K
PSPC1 regulates CHK1 phosphorylation through phase separation and participates in mouse oocyte maturation
1Hubei Provincial Key Laboratory of Developmentally Originated Disease, Department of Tissue and Embryology, School of Basic Medical Sciences, Wuhan University, Wuhan 430062, China.
Acta Biochimica Et Biophysica Sinica
|September 7, 2021
Summary
Paraspeckle component 1 (PSPC1) undergoes liquid-liquid phase separation (LLPS) to regulate mouse oocyte maturation. This process involves interaction with PPP5C to control CHK1 phosphorylation, crucial for germinal vesicle stage development.
Area of Science:
- Cell Biology
- Reproductive Biology
- Biochemistry
Background:
- Liquid-liquid phase separation (LLPS) is key for membraneless organelle formation in cells.
- The role of LLPS in mouse oocyte maturation is under-investigated.
- Paraspeckle component 1 (PSPC1) is implicated in tumor development but its function in oocyte maturation is unknown.
Purpose of the Study:
- To investigate the role of PSPC1 in mouse oocyte maturation.
- To determine if PSPC1's prion-like domain (PrLD) is essential for its function in oocytes.
- To elucidate the molecular mechanism by which PSPC1 influences oocyte maturation.
Main Methods:
- Sequence analysis to identify PSPC1's PrLD.
- In vitro phase separation assays for PSPC1.
- Immunofluorescence microscopy to localize PSPC1 in mouse oocytes.
- PSPC1 knockdown experiments.
- Co-immunoprecipitation (Co-IP) and Western blot analyses to study protein interactions and phosphorylation.
Main Results:
- PSPC1 undergoes LLPS, dependent on its PrLD.
- PSPC1 localizes to the germinal vesicle (GV) stage of mouse oocytes.
- PSPC1 knockdown impairs in vitro oocyte maturation.
- PSPC1 interacts with PPP5C and regulates CHK1 phosphorylation.
- PSPC1 lacking PrLD fails to regulate CHK1 phosphorylation, indicating LLPS is critical for function.
Conclusions:
- PSPC1 participates in mouse oocyte maturation through LLPS.
- PSPC1 regulates CHK1 phosphorylation via PPP5C, a process dependent on its phase separation ability.
- This study reveals a novel mechanism for LLPS in regulating mammalian oocyte development.
Related Concept Videos
02:17Separation of Sister Chromatids
3.9K
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
At the onset of anaphase, separase, a proteolytic enzyme, is...
3.9K
02:02Meiosis II
48.0K
Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
48.0K
02:19The Spindle Assembly Checkpoint
3.3K
The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
3.3K
02:15M-Cdk Drives Transition Into Mitosis
5.8K
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
5.8K
00:50Anaphase Promoting Complex
3.0K
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
3.0K
01:28The Cell Cycle Control System
4.1K
The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
4.1K